4GJX

Crystal structure of CD23 lectin domain mutant D258A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	CCD
Collection date	2011-01-21
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9763
Spacegroup name	P 65
Unit cell lengths	90.970, 90.970, 351.681
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	58.670 - 2.800
R-factor	0.2099
Rwork	0.208
R-free	0.25120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2h2r
RMSD bond length	0.006
RMSD bond angle	0.987
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASES
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.670	2.950
High resolution limit [Å]	2.800	2.800
Number of reflections	36967
Completeness [%]	91.6	94.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	291	18% ethanol, 4% PEG 400, 0.1 M sodium acetate pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K