4GJP

Crystal structure of the TAL effector dHax3 bound to dsDNA containing repetitive methyl-CpG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2012-04-08
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9778
Spacegroup name	P 1 21 1
Unit cell lengths	81.203, 87.114, 88.147
Unit cell angles	90.00, 102.85, 90.00

Refinement procedure

Resolution	39.441 - 1.940
R-factor	0.2017
Rwork	0.201
R-free	0.22450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3v6t
RMSD bond length	0.009
RMSD bond angle	1.289
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	1.940
Number of reflections	87970
Completeness [%]	99.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	291	10% PEG 3350, 14% ethanol, 0.1M MES pH6.2 , VAPOR DIFFUSION, HANGING DROP, temperature 291K