4GJH

Crystal Structure of the TRAF domain of TRAF5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	77
Detector technology	CCD
Collection date	2010-06-06
Detector	ADSC QUANTUM 210r
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	71.052, 90.579, 117.216
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.073 - 2.805
R-factor	0.227
Rwork	0.224
R-free	0.27310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.156
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MLPHARE
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.850
High resolution limit [Å]	2.800	7.590	2.800
Number of reflections	17854
Completeness [%]	93.8	98	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	289	0.1M Bis-Tris, 15%(w/v) PEG10000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K