4GIQ
Crystal Structure of mouse RANK bound to RANKL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-08-06 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 63 |
| Unit cell lengths | 120.636, 120.636, 94.297 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.740 - 2.700 |
| R-factor | 0.191 |
| Rwork | 0.188 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jtz |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.597 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 | 2.870 |
| High resolution limit [Å] | 2.700 | 4.890 | 2.700 |
| Rmerge | 0.080 | 0.042 | 0.537 |
| Number of reflections | 21040 | ||
| <I/σ(I)> | 13.9 | ||
| Completeness [%] | 98.1 | 99.8 | 92.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 2.0 M sodium chloride, 100 mM EDTA, 100 mM sodium acetate, pH 4.6, vapor diffusion, hanging drop, temperature 293K, VAPOR DIFFUSION, HANGING DROP |
