4GIM

Crystal Structure of Pseudouridine Monophosphate Glycosidase Complexed with Pseudouridine 5'-phosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-03-12
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9180
Spacegroup name	P 21 21 21
Unit cell lengths	60.657, 76.511, 199.162
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.331 - 1.802
R-factor	0.1787
Rwork	0.177
R-free	0.20720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vkm
RMSD bond length	0.007
RMSD bond angle	1.222
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (1.7.1_743)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.860
High resolution limit [Å]	1.800	3.880	1.800
Rmerge	0.089	0.060	0.565
Number of reflections	86181
<I/σ(I)>	8.6
Completeness [%]	99.9	98.8	100
Redundancy	4.9	4.7	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	20% PEG4000, 0.1 M Tris, 0.2 M sodium chloride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K