4GIA
Crystal structure of the MUTB F164L mutant from crystals soaked with isomaltulose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97559 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.100, 86.500, 118.600 |
| Unit cell angles | 90.00, 97.70, 90.00 |
Refinement procedure
| Resolution | 14.950 - 2.010 |
| R-factor | 0.1646 |
| Rwork | 0.162 |
| R-free | 0.21280 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1zjb |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.429 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.422 | |
| Number of reflections | 84263 | |
| <I/σ(I)> | 11.1 | 3.2 |
| Completeness [%] | 97.1 | 81.3 |
| Redundancy | 4 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 292 | 0.1M Tris-HCl, 12%(w/v) PEG20000, 0.01M L-Cysteine, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
