4GHL
Structural Basis for Marburg virus VP35 mediate immune evasion mechanisms
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793376 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 83.771, 93.384, 102.071 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.870 - 2.020 |
| R-factor | 0.18999 |
| Rwork | 0.187 |
| R-free | 0.23994 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fke |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.496 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.040 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmerge | 0.094 | 0.928 |
| <I/σ(I)> | 22.9 | 2.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.1M sodium citrate (pH 5.5), 0.1M sodium citrate (pH 6.0), 0.25% glycerol, 14% PEG 6000 and 0.6M ammonium sulfate., VAPOR DIFFUSION, HANGING DROP, temperature 293K |
