4GH4

Crystal Structure of Foot and Mouth Disease Virus A22 Serotype

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	294
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS II
Spacegroup name	I 2 2 2
Unit cell lengths	328.200, 341.800, 363.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 3.000
Rwork	0.167
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.652
Data reduction software	MOSFLM
Data scaling software	ROTAVATA
Phasing software	X-PLOR
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.000
Rmerge	0.179
Number of reflections	251190
Completeness [%]	61.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.6	294	3.8-4.5% PEG20000, 2.5 M ammonium chloride, 4% 1,4-dioxane, 0.1 M sodium phosphate, pH 7.6, VAPOR DIFFUSION, SITTING DROP, temperature 294K
1	VAPOR DIFFUSION, SITTING DROP	7.6	294	3.8-4.5% PEG20000, 2.5 M ammonium chloride, 4% 1,4-dioxane, 0.1 M sodium phosphate, pH 7.6, VAPOR DIFFUSION, SITTING DROP, temperature 294K