4GH0
Crystal structure of D48V mutant of human GLTP bound with 12:0 monosulfatide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-14 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9754 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 77.984, 47.242, 62.193 |
Unit cell angles | 90.00, 125.52, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.350 |
R-factor | 0.15359 |
Rwork | 0.152 |
R-free | 0.18078 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3s0i |
RMSD bond length | 0.014 |
RMSD bond angle | 1.602 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.400 |
High resolution limit [Å] | 1.350 | 1.350 |
Number of reflections | 40558 | |
Completeness [%] | 99.8 | 99.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 15% PEG 3350, 0.1M MES pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |