4GGT
Structure of apo Bradavidin2 (Form B)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-04-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 |
| Unit cell lengths | 34.771, 46.118, 46.449 |
| Unit cell angles | 106.59, 106.92, 106.82 |
Refinement procedure
| Resolution | 40.660 - 1.693 |
| R-factor | 0.21473 |
| Rwork | 0.212 |
| R-free | 0.25972 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ew1 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.677 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.693 |
| Number of reflections | 23010 |
| Completeness [%] | 86.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 3 | 293 | 1 uL drop of 3.4 mg/mL protein in 0.5 M acetic acid, pH 3.0, with 0.12 M magnesium formate, diffraction parameters improved with later addition of 0.1 M sodium bromide, MICROBATCH, temperature 293K |
