4GEQ

Crystal structure of the Spc24-Spc25/Cnn1 binding interface

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-10-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.127
Spacegroup name	P 21 21 21
Unit cell lengths	34.142, 61.848, 139.056
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.090 - 2.010
R-factor	0.2092
Rwork	0.207
R-free	0.25800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ftx
RMSD bond length	0.010
RMSD bond angle	0.970
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.850	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.113	0.577
Number of reflections	19858
<I/σ(I)>	8.5	2.5
Completeness [%]	96.9	87.1
Redundancy	4.6	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.6	293.15	15% PEG6000, 5% glycerol; Drop volume: 0.2ul; Protein proportion: 50%; Protein concentration: 6 mg/ml, pH 7.6, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K