4GEO
P38a MAP kinase DEF-pocket penta mutant (M194A, L195A, H228A, I229A, Y258A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.937, 74.658, 74.802 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.850 - 1.660 |
| R-factor | 0.21983 |
| Rwork | 0.218 |
| R-free | 0.25542 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.498 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 52.850 |
| High resolution limit [Å] | 1.660 |
| Number of reflections | 44163 |
| Completeness [%] | 98.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.25 | 277 | 15% w/v PEG3350, 0.1 M HEPES, pH 7.25, 0.2 M potassium fluoride, 25 mM beta-octyl glucoside, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
