4GEC
Crystal Structure of E.coli MenH R124A Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2010-12-15 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.953692 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 115.144, 115.144, 469.775 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 81.420 - 2.500 |
| R-factor | 0.1951 |
| Rwork | 0.194 |
| R-free | 0.22250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.060 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | MOLREP |
| Refinement software | BUSTER-TNT |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 117.444 | 81.419 | 2.640 |
| High resolution limit [Å] | 2.500 | 7.910 | 2.500 |
| Rmerge | 0.190 | 0.027 | 1.847 |
| Total number of observations | 25646 | 118624 | |
| Number of reflections | 54795 | ||
| <I/σ(I)> | 17.6 | 77.8 | 1.8 |
| Completeness [%] | 99.4 | 99.7 | 99 |
| Redundancy | 14.9 | 13.2 | 15.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | Sodium Citrate, Ammonium sulfate, Lithium sulfate, pH 5.5, vapor diffusion, hanging drop, temperature 291K |
