4GE0
Schizosaccharomyces pombe DJ-1 T114P mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-06-06 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.486, 51.743, 82.749 |
| Unit cell angles | 89.10, 89.08, 66.86 |
Refinement procedure
| Resolution | 36.510 - 1.450 |
| R-factor | 0.18014 |
| Rwork | 0.179 |
| R-free | 0.20772 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4GDH |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.241 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.6.0116) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.000 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.066 | 0.206 |
| Number of reflections | 113462 | |
| <I/σ(I)> | 19.9 | 5.8 |
| Completeness [%] | 94.5 | 84.9 |
| Redundancy | 5.1 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 26% PEG4000, 100 mM Tris-HCl, 200 mM magnesium chloride, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
