4GDO
Structure of a fragment of the rod domain of plectin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR-H |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-04-01 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.620, 35.870, 75.690 |
| Unit cell angles | 90.00, 94.11, 90.00 |
Refinement procedure
| Resolution | 45.194 - 1.700 |
| R-factor | 0.2148 |
| Rwork | 0.213 |
| R-free | 0.24840 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.870 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | Arcimboldo |
| Refinement software | PHENIX ((phenix.refine: dev_1117)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.000 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 23087 | |
| <I/σ(I)> | 3.52 | |
| Completeness [%] | 85.3 | 68.9 |
| Redundancy | 3.1 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 298 | 0.1M sodium acetate, 2.2M sodium chloride, 0.2M lithium sulfate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
