4GDA
Circular Permuted Streptavidin A50/N49
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-06-15 |
| Wavelength(s) | 0.78 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 46.809, 94.027, 104.279 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.450 - 1.000 |
| R-factor | 0.1195 |
| Rwork | 0.118 |
| R-free | 0.14320 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.574 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 14.456 | 14.456 | 0.990 |
| High resolution limit [Å] | 0.970 | 2.630 | 0.970 |
| Rmerge | 0.076 | 0.048 | 0.997 |
| Number of reflections | 135318 | ||
| <I/σ(I)> | 20.671 | ||
| Completeness [%] | 99.8 | 98.1 | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 25% saturated ammonium sulfate, 0.1 M Tris-chloride, 0.2 M lithium sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
