4GCN
N-terminal domain of stress-induced protein-1 (STI-1) from C.elegans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.212, 64.199, 124.933 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.900 - 1.850 |
| R-factor | 0.1803 |
| Rwork | 0.178 |
| R-free | 0.23290 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.676 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 34.900 | 50.000 | 1.880 |
| High resolution limit [Å] | 1.850 | 5.020 | 1.850 |
| Rmerge | 0.079 | 0.056 | 0.585 |
| Number of reflections | 25569 | ||
| <I/σ(I)> | 9.2 | 2.37 | |
| Completeness [%] | 97.0 | 99.4 | 74 |
| Redundancy | 7.6 | 7.5 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 0.2 M sodium chloride, 0.1 M Bis-Tris buffer, 25% PEG-3350, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
