4GBY

The structure of the MFS (major facilitator superfamily) proton:xylose symporter XylE bound to D-xylose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2011-10-10
Detector	RAYONIX MX225HE
Wavelength(s)	1.0000
Spacegroup name	P 43 21 2
Unit cell lengths	95.290, 95.290, 168.181
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.714 - 2.808
R-factor	0.2298
Rwork	0.228
R-free	0.27360
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.280
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.870
High resolution limit [Å]	2.770	2.770
Number of reflections	19996
Completeness [%]	98.4	96.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.6	291	40 % (w/v) PEG400, 0.05 M Glycine pH 9.6 and 0.1 M LiCl, VAPOR DIFFUSION, HANGING DROP, temperature 291K