4GB3
Human coxsackievirus B3 strain RD coat protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-02-07 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | H 3 2 |
Unit cell lengths | 296.638, 296.638, 813.215 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 15.000 - 2.740 |
R-factor | 0.33 |
Rwork | 0.330 |
R-free | 0.33900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1cov |
RMSD bond length | 0.008 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | CNS (1.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.840 |
High resolution limit [Å] | 2.740 | 2.740 |
Rmerge | 0.127 | 0.488 |
Number of reflections | 343901 | |
<I/σ(I)> | 8.75 | 2.97 |
Completeness [%] | 96.9 | 98 |
Redundancy | 4.4 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | 298 | 2 M ammonium sulfate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |