4GA5
Crystal structure of AMP phosphorylase C-terminal deletion mutant in the apo-form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-03 |
| Detector | RIGAKU SATURN A200 |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 124.330, 249.482, 162.369 |
| Unit cell angles | 90.00, 110.44, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.250 |
| Rwork | 0.261 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ga4 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.370 |
| High resolution limit [Å] | 3.250 | 3.250 |
| Number of reflections | 73009 | |
| <I/σ(I)> | 2.5 | |
| Completeness [%] | 99.8 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.1M citrate-NaOH, 12% (w/v) PEG 4000, 5% (v/v) ethylene glycol, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
