4G8W

Rat Heme Oxygenase-1 in complex with Heme and O2 with 13 hr illumination: Laser on

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2012-04-25
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 32 2 1
Unit cell lengths	65.300, 65.300, 121.465
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.760 - 2.400
R-factor	0.16868
Rwork	0.166
R-free	0.22833
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	4g8u
RMSD bond length	0.016
RMSD bond angle	2.223
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	REFMAC (5.6.0117)
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.440
High resolution limit [Å]	2.400	2.400
Number of reflections	12112
<I/σ(I)>	9.4
Completeness [%]	98.7	99.2
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	4M sodium formate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K