4G82

Crystal Structure of p73 DNA-Binding Domain Tetramer bound to a Full Response-Element

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-11
Detector	ADSC QUANTUM 315r
Spacegroup name	P 61
Unit cell lengths	175.472, 175.472, 34.317
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.870 - 3.100
R-factor	0.24
Rwork	0.238
R-free	0.26800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3vd0
RMSD bond length	0.012
RMSD bond angle	2.157
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.100
Rmerge	0.078
Number of reflections	11516
<I/σ(I)>	16.7
Completeness [%]	99.7
Redundancy	8.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		9	298	0.1M TRIS, 20% PEG3350 , pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K