4G7Z
Crystal structure of Thermus thermophilus transcription initiation complex containing 5-BrU at template-strand position +1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-04 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9180 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 185.465, 103.590, 296.507 |
| Unit cell angles | 90.00, 98.53, 90.00 |
Refinement procedure
| Resolution | 49.846 - 3.815 |
| R-factor | 0.177 |
| Rwork | 0.176 |
| R-free | 0.23220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4g7h |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.163 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.870 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmerge | 0.174 | 0.553 |
| Number of reflections | 97590 | |
| <I/σ(I)> | 9.5 | 2.1 |
| Completeness [%] | 89.4 | 46.7 |
| Redundancy | 6.4 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.7 | 295 | 100 mM Tris-HCl, pH 8.7, 200 mM potassium chloride, 50 mM magnesium chloride, 10% PEG4000, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
