4G72
Structure of Recombinant Cytochrome ba3 Oxidase mutant V236M from Thermus thermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 143.935, 98.436, 94.565 |
| Unit cell angles | 90.00, 127.87, 90.00 |
Refinement procedure
| Resolution | 74.650 - 3.190 |
| R-factor | 0.16632 |
| Rwork | 0.163 |
| R-free | 0.22274 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.328 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 74.650 |
| High resolution limit [Å] | 3.190 |
| Number of reflections | 16505 |
| Completeness [%] | 99.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 6.5 | 293 | 50mM Sodium Cacodylate PH 6.5, 1.6M NaCl, 40% PEG400 , Lipidic Cubic Phase, temperature 293K |
