4G6V

CdiA-CT/CdiI toxin and immunity complex from Burkholderia pseudomallei

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-06-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.92
Spacegroup name	F 2 2 2
Unit cell lengths	151.963, 173.654, 174.822
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.400 - 2.640
R-factor	0.2065
Rwork	0.204
R-free	0.24520
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.186
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.400	2.740
High resolution limit [Å]	2.640	2.640
Number of reflections	34071
Completeness [%]	99.5	100
Redundancy	29.6	30.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	298	0.49 M sodium phosphate monobasic, 0.96 M potassium phosphate dibasic, 10 mM triethylene glycol, 504 nM chymotrypsin, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K