4G6A

Structure of the Hepatitis C virus envelope glycoprotein E2 antigenic region 412-423 bound to the broadly neutralizing antibody AP33

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-12-02
Detector	PSI PILATUS 6M
Wavelength(s)	0.98000
Spacegroup name	P 1 21 1
Unit cell lengths	44.875, 191.804, 59.374
Unit cell angles	90.00, 111.08, 90.00

Refinement procedure

Resolution	47.970 - 2.501
R-factor	0.1803
Rwork	0.178
R-free	0.21950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4dgv
RMSD bond length	0.003
RMSD bond angle	0.786
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.2_869))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.970	2.540
High resolution limit [Å]	2.500	2.500
Number of reflections	29450
Completeness [%]	91.3	61.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	293	17% (w/v) PEG 4000, 8.5% (v/v) 2-propanol, 15% (v/v) glycerol and 0.085 M Sodium HEPES, pH 7.5, VAPOR DIFFUSION, temperature 293K