4G69

Structure of the Human Discs Large 1 PDZ2 - Adenomatous Polyposis Coli Cytoskeletal Polarity Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-03-08
Detector	ADSC QUANTUM 210
Wavelength(s)	1.127
Spacegroup name	P 21 21 21
Unit cell lengths	27.109, 51.184, 71.756
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.379 - 2.000
R-factor	0.1723
Rwork	0.171
R-free	0.18330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fe5
RMSD bond length	0.007
RMSD bond angle	0.980
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.6.1_357))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.000	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	6846
<I/σ(I)>	20.7	4.8
Completeness [%]	97.8	82.9
Redundancy	4	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.25	300	Protein solution: 1.4 mM Dlg1 PDZ2, 1.7 mM APC, 100 mM NaCl, 25 mM Tris pH 7.0, 0.1% B-ME Well: 200 mM sodium acetate, 100 mM sodium cacodylate pH 6.25, 28% PEG 8000 Drop = 1:1 protein:well solution , VAPOR DIFFUSION, HANGING DROP, temperature 300K