4G54
Crystal structure of a periplasmic domain of the EpsAB fusion protein of the Vibrio vulnificus type II secretion system
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 87 |
| Detector technology | CCD |
| Collection date | 2012-01-28 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 128.821, 44.523, 51.040 |
| Unit cell angles | 90.00, 110.59, 90.00 |
Refinement procedure
| Resolution | 47.780 - 1.550 |
| R-factor | 0.17615 |
| Rwork | 0.175 |
| R-free | 0.20223 |
| Structure solution method | SAD |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.307 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.590 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.056 | |
| Number of reflections | 36885 | |
| Completeness [%] | 93.0 | 93.2 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 220 mM Calcium Chloride, 20% PEG3350, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
