4G4E
Crystal structure of the L88A mutant of HslV from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6C1 |
| Synchrotron site | PAL/PLS |
| Beamline | 6C1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2009-12-08 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.24 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.275, 167.122, 90.313 |
| Unit cell angles | 90.00, 105.86, 90.00 |
Refinement procedure
| Resolution | 49.752 - 2.888 |
| R-factor | 0.2589 |
| Rwork | 0.258 |
| R-free | 0.29340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e94 |
| RMSD bond length | 0.100 |
| RMSD bond angle | 1.130 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.950 |
| High resolution limit [Å] | 2.888 | 2.900 |
| Number of reflections | 45193 | |
| Completeness [%] | 92.7 | 92.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 294 | 0.1M Tris-HCl, 28%(v/v) PEG MME550, 0.2M ammonium formate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
