4G3K
Crystal structure of a. aeolicus nlh1 gaf domain in an inactive state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-21 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 119.350, 119.350, 60.290 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.910 - 3.050 |
| R-factor | 0.212 |
| Rwork | 0.208 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.582 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.914 | 3.130 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Rmerge | 0.101 | 1.392 |
| Number of reflections | 8699 | |
| <I/σ(I)> | 1.7 | |
| Completeness [%] | 99.3 | 99.8 |
| Redundancy | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.2 | 293 | 32.5% PEG 3350, 250 mM NaCl, and 50 mM MES pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
