4G2A

Crystal structure of a putative nutrient binding protein (lpg2210) from Legionella pneumophila subsp. pneumophila str. Philadelphia 1 at 2.33 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-05-31
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9794,0.9795,0.9184
Spacegroup name	P 21 21 21
Unit cell lengths	37.953, 87.716, 99.766
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.362 - 2.330
R-factor	0.1702
Rwork	0.168
R-free	0.22170
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.070
Data reduction software	XDS
Data scaling software	XSCALE (March 15, 2012)
Phasing software	SHELX
Refinement software	BUSTER-TNT (2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.362	43.362	2.410
High resolution limit [Å]	2.330	5.000	2.330
Rmerge	0.090	0.051	0.679
Number of reflections	14552	1542	1343
<I/σ(I)>	10.09	21.7	1.85
Completeness [%]	97.5	93.2	97.5
Redundancy	3.44		3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	2.0M ammonium sulfate, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K