4G27
Calcium-calmodulin complexed with the calmodulin binding domain from a small conductance potassium channel splice variant and phenylurea
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 12.3.1 |
Synchrotron site | ALS |
Beamline | 12.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-12-08 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.12 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 76.460, 66.870, 65.130 |
Unit cell angles | 90.00, 93.31, 90.00 |
Refinement procedure
Resolution | 39.072 - 1.650 |
R-factor | 0.1928 |
Rwork | 0.191 |
R-free | 0.22820 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.264 |
Data reduction software | XDS ((xia2)) |
Data scaling software | XDS ((xia2) |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.690 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.035 | 0.645 |
Number of reflections | 39245 | |
<I/σ(I)> | 22.5 | 2.3 |
Completeness [%] | 99.5 | 99.7 |
Redundancy | 5 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 1.25 M lithium sulfate, 0.5 M ammonium sulfate, 0.1 M sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |