4G1I
Structure of the PrgH periplasmic domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2011-05 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97949 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 264.432, 33.380, 48.530 |
| Unit cell angles | 90.00, 91.60, 90.00 |
Refinement procedure
| Resolution | 45.957 - 1.850 |
| R-factor | 0.1758 |
| Rwork | 0.174 |
| R-free | 0.21390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gr0 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.545 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.048 | 0.495 |
| Number of reflections | 43189 | |
| <I/σ(I)> | 14.9 | 2.8 |
| Completeness [%] | 99.5 | 99.9 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 100 mM Bicine, pH 8.5, 20% PEG6000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
