4G12
Crystal structure of putative TetR family transcriptional regulator, Fad35R, from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-24 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97755 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 76.836, 83.211, 95.328 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.440 |
| R-factor | 0.21301 |
| Rwork | 0.208 |
| R-free | 0.25596 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.862 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX (Suite (CCP4)) |
| Refinement software | REFMAC (5.6.0116) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.500 |
| High resolution limit [Å] | 3.440 | 3.440 |
| Rmerge | 0.122 | 0.529 |
| Number of reflections | 15712 | |
| <I/σ(I)> | 12.4 | 2.54 |
| Completeness [%] | 99.9 | 98 |
| Redundancy | 4.3 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.2M tri-Sodium citrate, 0.1M Tris-HCl pH 8.5, 30%(v/v) PEG400, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
