4G0B
Structure of native HCT from Coffea canephora
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-05 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.873 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 116.100, 116.100, 158.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.000 |
| R-factor | 0.1893 |
| Rwork | 0.186 |
| R-free | 0.25004 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bgh |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.560 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0116) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.166 | 0.566 |
| Number of reflections | 22073 | |
| <I/σ(I)> | 8.5 | 3.1 |
| Completeness [%] | 99.0 | 98.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 1.6 M magnesium sulfate and 0.1 M MES, VAPOR DIFFUSION, SITTING DROP, temperature 293K, pH 6.5 |
