4G04
High-resolution Crystal Structural Variance Analysis between Recombinant and Wild-type Human Serum Albumin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 |
| Unit cell lengths | 58.745, 58.664, 95.726 |
| Unit cell angles | 87.85, 75.92, 73.47 |
Refinement procedure
| Resolution | 47.365 - 2.301 |
| R-factor | 0.2431 |
| Rwork | 0.240 |
| R-free | 0.31660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ao6 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.159 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.365 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.095 | |
| Number of reflections | 52509 | |
| <I/σ(I)> | 22.48 | |
| Completeness [%] | 90.6 | 90.6 |
| Redundancy | 34 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 291 | 28-30% (w/v), PEG3350, Sigma-Aldrich), 50mM potassium phosphate, pH 7.0, VAPOR DIFFUSION, temperature 291K |
