4FZW
Crystal Structure of the PaaF-PaaG Hydratase-Isomerase Complex from E.coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2010-09-14 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 131.976, 131.976, 153.865 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.793 - 2.550 |
| R-factor | 0.2818 |
| Rwork | 0.280 |
| R-free | 0.32110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.214 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.590 |
| High resolution limit [Å] | 2.550 | 6.920 | 2.550 |
| Rmerge | 0.107 | 0.056 | 0.485 |
| Number of reflections | 51031 | ||
| <I/σ(I)> | 9.5 | ||
| Completeness [%] | 99.8 | 97.4 | 100 |
| Redundancy | 8 | 7.7 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.1 M Sodium Citrate pH 5.5, 0.2 M Sodium acetate, 5% (w/w) PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
