4FZR

Crystal Structure of SsfS6, Streptomyces sp. SF2575 glycosyltransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-02
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.12715
Spacegroup name	P 61 2 2
Unit cell lengths	82.605, 82.605, 230.516
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.763 - 2.397
R-factor	0.226
Rwork	0.224
R-free	0.26170
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.861
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX (PHASER)
Refinement software	PHENIX (1.8_1063)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.440
High resolution limit [Å]	2.400	6.510	2.400
Rmerge	0.094
Number of reflections	19017
<I/σ(I)>	8.7
Completeness [%]	99.0	88.9	92.2
Redundancy	11.5	8.9	10.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.2	298	Protein Solution (11.4 mg/ml SsfS6 protein, 50mM Tris pH 8.0) mixed in a 1:1 ratio with the well solution (1.13~1.40 M Sodium phosphate monobasic monohydrate / Potassium phosphate dibasic, pH 8.2) Cryoprotected with 100% Paratone-N, VAPOR DIFFUSION, SITTING DROP, temperature 298K
1	VAPOR DIFFUSION, SITTING DROP	8.2	298	Protein Solution (11.4 mg/ml SsfS6 protein, 50mM Tris pH 8.0) mixed in a 1:1 ratio with the well solution (1.13~1.40 M Sodium phosphate monobasic monohydrate / Potassium phosphate dibasic, pH 8.2) Cryoprotected with 100% Paratone-N, VAPOR DIFFUSION, SITTING DROP, temperature 298K