4FZG
20S yeast proteasome in complex with glidobactin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-10-06 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 134.770, 300.350, 144.490 |
| Unit cell angles | 90.00, 112.67, 90.00 |
Refinement procedure
| Resolution | 15.000 - 3.000 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.22600 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.067 | 0.472 |
| Number of reflections | 209581 | |
| <I/σ(I)> | 15.2 | 3.2 |
| Completeness [%] | 99.3 | 99.7 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 293 | 100 mM MES; 20 mM MgAc2; 12% , pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
