4FZF
Crystal structure of MST4-MO25 complex with DKI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-09-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97915 |
Spacegroup name | I 4 3 2 |
Unit cell lengths | 239.464, 239.464, 239.464 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.850 - 3.640 |
R-factor | 0.25515 |
Rwork | 0.252 |
R-free | 0.30916 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | : 1UPL and 3GGF |
RMSD bond length | 0.007 |
RMSD bond angle | 1.323 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.710 |
High resolution limit [Å] | 3.640 | 3.640 |
Number of reflections | 13356 | |
<I/σ(I)> | 4.1 | |
Completeness [%] | 99.6 | 100 |
Redundancy | 12.8 | 13.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 289 | 0.1M Tris pH 8.0, 20% PEG 350 mme, VAPOR DIFFUSION, HANGING DROP, temperature 289K |