4FZC
20S yeast proteasome in complex with cepafungin I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-10-06 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 135.680, 301.020, 144.830 |
Unit cell angles | 90.00, 112.86, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.800 |
R-factor | 0.214 |
Rwork | 0.214 |
R-free | 0.24400 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.077 | 0.614 |
Number of reflections | 256455 | |
<I/σ(I)> | 13 | 2.4 |
Completeness [%] | 97.8 | 99 |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 293 | 100 mM MES; 20 mM MgAc2; 12% MPD, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |