4FZ0

Crystal structure of acid-sensing ion channel in complex with psalmotoxin 1 at low pH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-02
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	232.290, 108.710, 126.360
Unit cell angles	90.00, 119.78, 90.00

Refinement procedure

Resolution	38.373 - 2.800
R-factor	0.2035
Rwork	0.202
R-free	0.23140
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.120
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.800	2.800
Number of reflections	63727
Completeness [%]	99.9	99.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	297	100 mM Sodium Acetate, 9-12% PEG 2000 MME, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K