4FYH

Crystal structure of rcl with phospho-triciribine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-10-22
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9334
Spacegroup name	P 21 21 2
Unit cell lengths	92.953, 119.002, 57.769
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.500 - 2.440
R-factor	0.21093
Rwork	0.209
R-free	0.24645
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.093
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		2.570
High resolution limit [Å]	2.440	2.440
Rmerge		0.376
<I/σ(I)>		5
Completeness [%]	100.0	100
Redundancy		7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.6	291	100 mM Tris, 1.2 Li2SO4, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 291.K