4FXX

Structure of SF1 coiled-coil domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-12-15
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.9794
Spacegroup name	C 1 2 1
Unit cell lengths	96.030, 37.970, 144.680
Unit cell angles	90.00, 107.38, 90.00

Refinement procedure

Resolution	34.966 - 2.480
R-factor	0.2393
Rwork	0.236
R-free	0.26980
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	0.870
Data reduction software	MOSFLM
Data scaling software	SCALA (CCP4_3.2.19)
Phasing software	PHENIX (1.7.1_743)
Refinement software	PHENIX (1.7.1_743)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.000	48.000	2.610
High resolution limit [Å]	2.480	7.840	2.480
Rmerge	0.108	0.080	0.370
Total number of observations		3158	10355
Number of reflections	16211
<I/σ(I)>	10.5645
Completeness [%]	89.2	97.59	75.39
Redundancy	5.08	5.13	5.29

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	277	1.34 M sodium malonate pH 6.0, 0.1 M imidazole maleate pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K