4FXD

Crystal structure of yeast DNA polymerase alpha bound to DNA/RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-11
Detector	ADSC QUANTUM 210
Wavelength(s)	0.93340
Spacegroup name	P 1
Unit cell lengths	72.140, 74.770, 116.990
Unit cell angles	82.26, 72.57, 82.40

Refinement procedure

Resolution	55.526 - 3.000
R-factor	0.2555
Rwork	0.254
R-free	0.28480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fvm
RMSD bond length	0.003
RMSD bond angle	0.766
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1078))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.770	3.160
High resolution limit [Å]	3.000	3.000
Rmerge	0.073	0.921
Number of reflections	45027
<I/σ(I)>	13.6	1.5
Completeness [%]	97.8	97.5
Redundancy	3.9	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	0.1 M Bicine, 12% PEG3350, 10 mM MgCl2, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K