4FX8

Crystal structure of the mutant Q185A.R203A of orotidine 5'-monophosphate decarboxylase from Methanobacterium thermoautotrophicum complexed with inhibitor BMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-04-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.075
Spacegroup name	P 41 21 2
Unit cell lengths	91.511, 91.511, 135.376
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.774 - 1.941
R-factor	0.173
Rwork	0.172
R-free	0.19800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ltp
RMSD bond length	0.007
RMSD bond angle	1.004
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall
Low resolution limit [Å]	46.774
High resolution limit [Å]	1.941
Number of reflections	41820
Completeness [%]	96.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	2.0M Ammonium sulfate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K