4FX4

Crystal structure of M. tuberculosis transcriptional regulator MOSR (Rv1049) in compex with DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	77
Detector technology	CCD
Collection date	2011-07-05
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	H 3
Unit cell lengths	146.585, 146.585, 53.887
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.475 - 3.100
R-factor	0.2419
Rwork	0.240
R-free	0.28000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1z9c
RMSD bond length	0.003
RMSD bond angle	0.818
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.475	3.310
High resolution limit [Å]	3.100	3.100
Number of reflections	7799
Completeness [%]	99.6	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	298	1.4 M sodium phosphate monobasic monohydrate/potassium phosphate dibasic, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K