4FX0

Crystal structure of M. tuberculosis transcriptional regulator MosR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	77
Detector technology	CCD
Collection date	2011-03-30
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.033
Spacegroup name	P 21 21 21
Unit cell lengths	51.998, 66.677, 79.089
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.846 - 2.698
R-factor	0.2326
Rwork	0.231
R-free	0.27600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.579
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.850	2.770
High resolution limit [Å]	2.698	2.698
Number of reflections	7602
Completeness [%]	95.5	78.54

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	289	0.2 M SODIUM CITRATE, PH 5.5, 20% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 289K