4FWW
Crystal structure of the Sema-PSI extracellular domains of human RON receptor tyrosine kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2010-02-24 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0332 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 106.400, 73.300, 87.900 |
| Unit cell angles | 90.00, 97.40, 90.00 |
Refinement procedure
| Resolution | 19.320 - 1.850 |
| R-factor | 0.19311 |
| Rwork | 0.191 |
| R-free | 0.23245 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2UZX chain B |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.548 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.320 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.069 | 0.440 |
| Number of reflections | 51510 | |
| <I/σ(I)> | 10.46 | 1.47 |
| Completeness [%] | 90.0 | 63.7 |
| Redundancy | 2.8 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 19% PEG 4000, 0.1 M Sodium Acetate, pH 4.6, 0.2 M Ammonium Sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
