4FWU
Crystal structure of glutaminyl cyclase from drosophila melanogaster in space group I4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.542 |
| Spacegroup name | I 4 |
| Unit cell lengths | 122.344, 122.344, 72.736 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.000 - 2.000 |
| R-factor | 0.1695 |
| Rwork | 0.166 |
| R-free | 0.20790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4f9u |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.448 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.075 | 0.460 |
| Number of reflections | 35641 | |
| <I/σ(I)> | 11.9 | 2 |
| Completeness [%] | 98.0 | 93 |
| Redundancy | 3.5 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 1.6M ammonium sulphate, 10% (v/v) 1,4-dioxane, 0.1M HEPES, 40mM MEGA-9, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
